C. elegans PAR-3 and PAR-6 are required for apicobasal asymmetries associated with cell adhesion and gastrulation
Development
Nance et al. 130 (22): 5339.
Movie 1B
-
Ingression
of the E daughters.Movies are
video recordings of a single focal plane, beginning at the 24-cell stage and
terminating at the 46-cell stage of embryogenesis. Timepoints are 45 seconds
apart; elapsed time is 17 minutes. The E daughters are indicated by asterisks.
(A) Wild-type embryo. (B) par-3(ZF1) embryo. Note that the
wild-type E daughters divide after they complete ingression, while the par-3(ZF1) E daughters divide at the embryo surface.
Movie 2B
-
NMY-2GFP
localization during ingression of the E daughters.Movies are confocal images of GFP in wild-type (A)
or par-3(ZF1) (B) embryos expressing nmy-2::gfp, beginning at the 26-cell stage and terminating at
the 46-cell stage. Timepoints are 3 minutes apart; elapsed time is 18 minutes.
The E daughters are indicated by asterisks. Note that GFP accumulates at the
apical surfaces of wild-type, but not par-3(ZF1) E daughters. As in Movie 1, the wild-type E
daughters divide after ingression, while the par-3(ZF1) E daughters divide at the surface.
