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Fig. 2. Anterior-posterior asymmetry. (A-D) Wild-type, 2-cell embryo in the light
microscope (A) after staining for P granules (B, green), and PIE-1 (C, red).
DNA is shown in blue. (D) A 24-cell, wild-type embryo with an
end-1::gfp transgene; the GFP fluorescence image (green) was
superimposed on the light microscope image. The middle and bottom rows show
par-mutant embryos (middle) and par(ZF1) embryos (bottom)
prepared as above. Embryo genotypes are as follows: par-3(it71)
(E,F,H); par-6(zu222) (G); par-3(ZF1) (I,J,L);
par-6(ZF1) (K).
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