Fig. 5. Hh signaling is required for expression of ngn1 and neurod in DRG precursors. yot, smu and wild-type embryos were processed for in situ hybridization at 31-36 hpf to reveal ngn1 or neurod mRNA expression. (A) ngn1 shows broad expression in the spinal cord in addition to its DRG precursor expression (arrowheads), whereas (B) neurod expression in the trunk is restricted to DRG precursors (arrowheads). (C,D) In yot and smu embryos, spinal cord staining is maintained, but ngn1 and neurod expression in DRG precursors is eliminated.

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