Fig. 8. Transplanted wild-type (WT) DRG precursor cells rescue DRG development in smu and yot backgrounds. (A) Lateral view of a smu host labeled with anti-Hu antibody viewed by confocal microscopy. (B) WT cells are shown in red in the same optical section. (C) Photoshop merge of A and B showing co-localization of labeled WT cells and Hu+ DRG neurons. (D) Lateral views of a yot host embryo labeled with anti-Hu and anti-zn5 (both in green). Hu expression can be seen in the spinal cord and in two separate ganglia (arrows) lateral to the spinal cord. zn-5 expression can be seen in secondary motor neuron projections (arrowheads). Notice the motor neuron projections are truncated, consistent with previously reported outgrowth defects in yot mutants (Brand et al., 1996). (E) The same yot mutant host embryo shown in D showing WT donor-derived cells (red). (F) Photoshop merge of D and E showing co-localization of labeled WT cells and Hu+ DRG neurons.

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