DEV00758 Movies

Files in this Data Supplement:

• Movie 1 - Timelapse movie showing the movement of gsc-GFP positive cells (prechordal plate and notochordal precursor cells) in a wild-type embryo from shield stage to bud stage.
• Movie 2 - Timelapse movie showing the movement of epiblast (ectoderm, red – rhodamine) and hypoblast (prechordal plate, green – GFP) cells in wild type embryos for 45 minutes at the onset of gastrulation. Hypoblast (prechordal plate) cells are moving from bottom (germ ring) to the top (animal pole), while epiblast cells are moving in the opposite direction. Dorsal (start of movie), ventral (middle) and lateral (end) views.
• Movie 3 - Timelapse movie showing the movement of epiblast (ectoderm, red – rhodamine) and hypoblast (prechordal plate, green – GFP) cells in slb embryos for 45 minutes at the onset of gastrulation. Hypoblast (prechordal plate) cells are moving from bottom (germ ring) to the top (animal pole), while epiblast cells are moving in the opposite direction. Dorsal (start of movie), ventral (middle) and lateral (end) views.
• Movie 4 - Timelapse movie showing the movement of wild-type and slb prechordal plate precursor cells at four consecutive timepoints (0, 2.5, 5, 7.5 minutes). The cells move from top (germ ring) to bottom (animal pole).
• Movie 5 - Timelapse movie showing the movement of two wild-type and three slb epiblast cells at four consecutive timepoints (0, 2.5, 5, 7.5 minutes). The cells move from top (animal pole) to bottom (germ ring).