Fig. 2. Perturbing Egfr signalling disrupts ommatidial rotation. Upper panels show sections through adult eyes, lower panels are schematics of these images. (A,B) aos^w11 clone (note, in this section, the equator can not be seen, but runs left to right as in all other images); (C,D) S⁵⁶⁷¹/+; (E,F) ru¹; (G,H) DN-Egfr expressed under the control of HS-Gal4; (I,J) pnt¹²⁷⁷/pnt⁸⁸ (position of equator cannot be accurately determined because of the large proportion of mis-specified ommatidia). In all cases, misrotated ommatidia can be seen. (K) Graph showing rotational angles in wild type (blue), sev-Gal4, UAS-keren (red) and ru¹ (green) eyes. Data is plotted as percentage of ommatidia at each angle. In each case, 5-600 ommatidia were scored from 5-6 eyes. UAS-keren and ru¹ have qualitatively similar effects on rotation. (L-Q) roulette is allelic to argos. (L,M) rlt¹ mutants show similar phenotypes to Egfr pathway mutants (compare L with other images in Fig. 2). (N,O) The rlt¹ mutant fails to complement aos^w11. (P,Q) rlt¹ phenotype can be rescued by overexpression of one copy of the sev-argos transgene. Colour coding in schematics is as for Fig. 1 (black, correctly orientated; green, underrotated; blue, overrotated; red line, equator). Black circles indicate misspecified ommatidia in this and all subsequent Figures.

Return to article