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Fig. 4. Eye discs misexpressing Keren are indistinguishable from wild type (WT).
All images show eye discs taken from crawling third instar larvae. In all
cases, green is
-Elav, marking photoreceptors. Upper panels show the
red channel alone; lower panels are merges. (A D)
m
0.5-lacZ staining (red) in WT (A,B) and
UAS-keren (C,D) discs. m
0.5-lacZ highlights
the R4 cell and acts as a marker for chirality; R4 determination is normal in
UAS-keren discs. (E-H)
-Bar staining (red), highlighting R1
and R6 in WT (E,F) and UAS-keren (G,H) discs. Rare misrotations can
be seen in both WT and mutant discs (arrowheads). (I,L) svp-lacZ
(red) staining in WT (I,J) and UAS-keren (K,L) discs.
seven-up is expressed strongly in R3 and R4 (outer pair in each
ommatidium) and weakly in R1 and R6 (inner pair). Note the similarity between
WT and UAS-keren with all three markers.