Fig. 7. Expression of the atypical cadherin Flamingo is altered within the R3/R4 precursor pair in argos^rlt discs. Confocal images of third instar eye discs are shown, anterior is left. The morphogenetic furrow is at the left edge of each panel. (A,C,G) wild-type (WT) and (B,D,H) aos^rlt discs stained with anti-Fmi (green in A-D, black and white in A' and B'), anti-DE-cadherin (magenta in A,B and b/w in A'' and B'') and F-actin (red, labeled by Phalloidin, in C,D and b/w in C',D'). A higher magnification of the Fmi and DE-cadherin stainings in similar discs is shown in G and H (ommatidial rows 1-11). Note that Fmi, in WT downregulated in the R3 precursor by row 7 and subsequently detected only around R4, is still present in R3 cells in aos^rlt until rows 11-12 and beyond. Typical WT and mutant examples are highlighted with yellow arrowheads in A',B',G,H. Abnormal localization of Fmi does not correlate with R3/R4 cell-fate defects, as the R4-specific marker m-lacZ is restricted to R4 in WT (E) and aos^rlt mutant (F) preclusters.

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