Fig. 5. XKLC4 translocates during cortical rotation and associates with GBP-GFP in particles. (A,B) XKLC4-GFP particles exhibit fast transport opposite the yolk during cortical rotation. Shown here are time-lapse images of the vegetal shear zone of an activated egg showing movements of XKLC4-GFP (green) and yolk platelets (red) during peak cortical rotation. (A) First frame of sequence showing starting position of four XKLC4-GFP particles (yellow circles) and three yolk platelets (asterisks). (B) Same field of view approximately 38 seconds later showing paths (yellow arrows) taken by the XKLC4-GFP particles moving from left to right and yolk platelets (Y) moving from right to left. Please also see Movies 2-4 (http://dev.biologists.org/supplemental/) to view time-lapse movies of XKLC4-GFP particle translocation just prior to cortical rotation, during peak cortical rotation and during late cortical rotation. (C-E) Localization of XKLC4-HA and GBP-GFP during cortical rotation. (C) Confocal image of GBP-GFP particles in the vegetal shear zone of an egg fixed during peak cortical rotation. (D) Localization of XKLC4-HA stained with anti-HA antibodies and labeled with Alexa Fluor 568 in the same confocal section. (E) Merged images of C and D. Arrowheads indicate examples of particles containing both GBP-GFP and XKLC4-HA.

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