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Fig. 8. Disruption of the Dynein motor complex selectively interferes with FMRFa expression in Tv neurons. (A) FaRP expression in third instar larval brain and ventral ganglia in which {Delta}Gl is produced in neuroendocrine cells using the C929 driver. Note lack of staining in the NHO and Tv neurons, SE2 unaffected (light-blue arrows). (B) Expression of {Delta}Gl in the NHO using the 24B driver does not interfere with FaRP staining in Tv neurons (yellow arrows) or NHO (white arrow). (C) FaRP staining in animals expressing p50/Dynamitin using the C929 driver. P-Mad accumulation in the midgut of Y45>Gal4; UAS-{Delta}Gl (D) and wild-type embryos (E). P-Mad accumulation in future amnioserosa cells of da>Gal4; UAS-{Delta}Gl (F) and wild-type embryos (G). Scale bars: in A, 50 µm for A-C; in D, 200 µm for D-G. Anterior leftwards in all panels. (A-C,F,G) Dorsal views; (D,E) lateral views.





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