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Fig. 5. Dominant-negative eomes inhibits dorsal expression of gsc
and flh. (A-D) The dorsal blastoderm margin of embryos at 50% epiboly
was dissected and flat-mounted. Bottom right corner indicates in situ probe
used, top right corner indicates gene construct, if injected. (A) Expression
of gsc in an uninjected embryo. (B) Injection of
eomes-eng inhibits gsc expression. Eomes-eng
expressing cells shown in brown by anti-GFP antibody staining. (C) Expression
of flh in an uninjected embryo. (D) Injection of
eomes-eng inhibits flh expression. Eomes-eng
expressing cells shown in brown by anti-GFP staining.
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