Fig. 8. Overexpression of eomes in mutant embryos. All embryos are at 6 hpf (equivalent to the shield stage) with dorsal to the right and are animal-pole views, except K,L which are lateral views. (A-F) boz-mutant embryos. (G-L) MZoep-mutant embryos. (A,C,E,G,I,K) Uninjected embryos. (B,D,F,J,L) Embryos injected with myc-eomes. (H) An eomes-VP-injected embryo. Expression of gsc is shown in A,B,G,H, chd in C,D,I,J and flh in E,F,K,L. Arrowheads indicate regions of ectopic expression. (J) Two cells of an eight-cell-stage embryo were injected and stained with the anti-Myc antibody. Regions of Myc staining are indicated with an arrow and arrowhead. Expanded chd expression is only observed on the dorsal side (purple stain, arrowhead).

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