Supplemental Figure
-
Fig. S1.
Overexpression of PP2A:B56e
leads to anterior truncation without inhibiting dorsal specific gene expression
during gastrulation. (A) Control tadpoles from uninjected embryos. (B) Tadpoles
after injection of 100pg PP2A:B56e mRNA
into two dorsal blastomeres at the four-cell stage. Nineteen out of a total 37
injected embryos showed anterior truncation. Embryos injected with 100pg of
PP2A:B56e mRNA into both ventral
blastomeres at the four-cell stage develop normally, while injection of higher
dose of PP2A:B56e RNA inhibits cell
division (data not shown). (C) Chordin expression in a control stage 10 embryo.
(D) Chordin expression at stage 10 is unaffected by injection of PP2A:B56e mRNA. (E) The expression of siamois and Xnr3,
as determined by RT-PCR, is normal in PP2A:B56e-injected embryos at stage 10. (F) expression of otx2, but not
of en2 or wnt1, is reduced on the injected side in embryos injected with 100pg
of PP2A:B56e mRNA into one dorsal
blastomere at the four-cell stage (embryos were coinjected with lacZ mRNA as a lineage tracer).
is required for Wnt/ß-catenin signaling during embryonic development
