Fig. 4. Wnt signaling is required for dT-specific differentiation in response to zli-derived signals. (A) Diagram of experimental procedure. HH stage 17 zli explants were co-cultured with either prospective vT or dT explants from HH stage 8 embryos in the presence or absence of cki7 for 36-48 hours, fixed, and then processed by in situ hybridization. The horizontal line indicates the limit of the notochord and predicts the future location of the zli. (B) Co-culture induction data was quantified and analyzed statistically using a ² significance test. (C-J) Representative in situ hybridization images for Dlx2 (D,F,H,J) and Gbx2 (C,E,G,I) in the indicated co-culture conditions. Dotted lines represent the border between prospective dT or vT explants and zli explants, as determined by using CellTracker. The total number of explants in each experimental condition are indicated above each bar in B. *, P<0.05. Scale bar: 100 µm.

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