Fig. 5. Wnt is required in ovo for proper induction of Gbx2 and repression of Dlx2 in the diencephalon. (A-D) Adjacent sections were processed by in situ hybridization to visualize the expression of Gbx2 (A,C) and Dlx2 (B,D). Electroporation was used to misexpress Dkk1 in the developing diencephalon of two HH stage-9 embryos (C,D). Ectopic expression of Dkk1 resulted in ectopic expression of Dlx2 (arrow in D) in the normally Gbx2-positive dT (A). Arrowheads indicate the position of the zli. Scale bar: 100 µm.

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