Fig. 2. Generation of embryos lacking both bon and sur activity by two different approaches. (A) In the genetic approach, bon and sur heterozygous fish are crossed to generate bon and sur double heterozygotes, which are crossed to sur homozygous fish. The sur homozygous and bon heterozygous embryos are injected with sur mRNA to rescue sur mutants. The intercrossing of these fish results in embryos lacking both bon and maternal and zygotic sur. (B) In the morpholino approach, bonMO is injected into MZsur mutants at the one- to two-cell stage to give rise to embryos lacking both bon activity and maternal and zygotic sur.

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