Fig. 4. Roles of bon and sur in heart and nervous system development. Wild-type (A-C), bonMO (D-F), bon (G-I), MZsur (J-L), MZsur;bonMO (M-O) and MZsur;bon (P-R) embryos at 30 hpf. Ventral view of cardiac myosin light chain 1 (cmlc1) expression (A,D,G,J,M,P) in a single normal heart in wild-type (A), in two reduced hearts in bonMO (D) and bon (G), in a single heart in MZsur (J), but not in MZsur;bonMO (M) and MZsur;bon (P) embryos (arrows). Lateral view, anterior to the left, dorsal up, of emx1 expression in telencephalon (arrowhead in B,E,H,K,N,Q); normal emx1 expression in wild-type (B), bonMO (E), bon (H) and MZsur (K), and anterior expression in bulb-like structure in MZsur;bonMO (N) and MZsur;bon (Q). Expression of shh in head and trunk region (C,F,I,L,O,R), normal expression in wild-type (C), bon (F) and bonMO (I) but discontinuous weak expression in MZsur (L) and absence of staining in MZsur;bonMO (O) and MZsur;bon (R), indicating absence of ventral CNS. Also notice the expression in endoderm in wild-type and MZsur embryos.

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