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Fig. 9. Regulation of Smad2 phosphorylation by bon and sur. Western-blot analysis of wild-type, MZsur, bonMO and MZsur;bonMO embryos at dome and shield stage. At dome stage, MZsur and MZsur;bonMO embryos have lower phosphorylated Smad2 levels but bonMO embryos are not affected. Phosphorylated-Smad2 levels recover by shield stage. Detection of actin serves as loading control.





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