Fig. 3. Antisense Vg1 RBP oligonucleotides disrupt roof plate formation and neural crest migration. Transverse confocal and paraffin sections were obtained from tailbud stage embryos injected with CMO (A,A',C,E), AMO (B,B',D,F), or AMO with sense Vg1 RBP mRNA (G). For each experiment, the approximate plane of section is indicated. Neural crest cells, indicated by the arrows, are found dorsal to the neural tube in CMO-injected embryos (A,A',C), but are not detectable in the corresponding AMO-injected embryos (B,B',D). A' and B' are high power magnifications of A and B, respectively. By stage 34, pigmented melanophores are observable both above the roof plate and at lateral-ventral positions in embryos injected with either CMO (E), or with AMO and sense Vg1 RBP mRNA (G). In AMO-injected embryos at this stage (F), however, the only detectable pigment is in the dorsal half of the neural tube and is completely absent from the lateral or ventral regions. The position of the neural tube roof plate is indicated by an arrowhead; note that it is missing in the AMO-injected embryos (B,B',D,F), and normally formed in the `rescued' embryos (G). Apparently apoptotic cells (*) are observed in the lumen of AMO-injected, tailbud stage embryos (D,F,G). In A-D, the neural tube is outlined in red. no, notochord.

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