Fig. 8. NMY-1 and MEL-11 localization in myosin-depleted embryos. In mlc-4 embryos, NMY-1 collapsed into large foci (A), but MEL-11 was unaffected (B). A merged image is shown in (C, NMY-1 red, MEL-11 green). However, although LET-502 appeared normal (D), MEL-11 was clearly disrupted in nmy-1(sb113); nmy-1(RNAi) embryos (E, merged image shown in F with LET-502 red, MEL-11 green) where it remained cytoplasmic in comparison to its membrane location in wild-type embryos of similar stages (e.g. Fig. 5E). MEL-11 membrane localization was decreased compared with the normal pattern of LET-502 (G), or as shown in this embryo (H), occasionally absent when total NMY was only partially depleted in nmy-1(sb113) (I, merged image, LET-502 red, MEL-11 green). Scale bar: 10 µm.

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