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Fig. 6. The asymmetric distribution of GPR-1/2 and LET-99 in EMS cells is dependent on MES-1/SRC-1 signaling. Confocal images of wild-type (A-I), mom-5 and mes-1 (J), and let-99(or204ts) (K) four-cell embryos stained with anti-GPR-1/2 or anti-LET-99 antibodies (as indicated) and DAPI (lower panels). (A,D) EMS in interphase. (B,C,E,F,G-K) EMS in prophase. (G-I) EMS in prophase double stained with both GPR-1/2 and LET-99 antibodies. Merged image (I) shows LET-99 in green and GPR-1/2 in red. Scale bar: 10 µm.





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