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Fig. 7. G{alpha} and let-99 are required for nuclear rotation in EMS cells. DIC images of live wild-type (A-E), gpa-16(it143ts) (F-J) and let-99(or204ts) (K-O) embryos recorded by time-lapse video microscopy after shifting to 25°C as described in text. Black arrowheads mark the current position of centrosomes. Note the normal division of P1 (F,G,K,L), but the absence of EMS nuclear rotation in the mutants. Scale bar: 10 µm.





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