Fig. 4. Map position and predicted structure of the gpa-16/spn-1 gene. (A) Map of the left arm of LGI, showing selected markers and the telomere (red square). Numbers above the line indicate map units. Regions deleted by the hDf10 mutation and duplicated by the sDp2 duplication are indicated by horizontal lines below the map. gpa-16(it143) was positioned close to bli-3 by two- and three-factor mapping. (The src-1 locus, used for mapping but not shown, is located one map unit to the left of unc-35.) (B) Structure of the gpa-16 gene as determined by comparing cDNA sequence to the genomic sequence. The extent of the deletion pk481 and the position of the point mutation it143 are indicated in blue. PCR primers designated 1F, 5R and 6R (sequences available on request) were used to assay presence of the wild-type and the deletion sequence in single mutant animals by direct PCR sequencing (see Fig. S2 at http://dev.biologists.org/supplemental/ and text). The sequence around the GA substitution (bold) in the mutant is TTTTCGACGTTGACGGACAGCGATCC.

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