Fig. 6. Development of the peripheral nervous system in tfap2a. (A-F) Detection of PNS and CNS components by whole-mount immunohistochemistry with anti-Hu antibodies. (A,B) Dorsal view of the anterior intestine at 72 hpf in (A) wild-type and (B) tfap2a mutant embryos showing sympathetic (black arrows) and enteric neurons (white arrows). (C,D) Dorsal view of the posterior gut tube at 48 hpf. (D) In tfap2a mutant embryos no enteric neurons can be detected in the posterior gut tube. (E,F) Lateral view of the trunk region of (E) wild-type and (F) tfap2a mutant embryos 48 hpf: Rohan Beard sensory neurons (white arrows) and dorsal root ganglia (black arrows) can be detected in mutants, but the number of neurons is reduced in each group. (G,H) Whole-mount in situ hybridization with isl1 riboprobes revealing presence of sympathetic neurons (black arrows) in (G) wild-type and (H) tfap2a mutant embryos. (I,J) Visualization of sympathetic neurons (black arrows) in (I) wild-type and (J) tfap2a mutant embryos by whole-mount in situ hybridization with phox2a riboprobes. (A-J) anterior towards the left. no, notocord; sc, spinal cord.

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