Fig. 3. tfap2a expression in early zebrafish embryos. Whole-mount in situ hybridization was performed with an antisense probe to tfap2a. (A) Lateral and (B) animal pole views of expression in the non-neural ectoderm (nn) of an 8 hpf gastrula-stage embryo. Arrows indicate boundary with neural ectoderm. (C) Lateral and (D) dorsal views, anterior towards the left, of expression in the cranial neural crest at 12 hpf (D) and at nine somites (C). Arrowheads indicate premigratory spinal neural crest. (E) Lateral view, 24 hpf; higher magnification is shown in F of segmental expression in the hindbrain and pharyngeal arches. (G-I) Dorsal views of expression in successively more posterior regions of the CNS. (G) Expression in the telencephalon and mesencephalon. (H) Segmental clusters of neurons in each rhombomere. (I) Expression in Rohon-Beard cells in the dorsal spinal cord. (J,K) Lateral views of tfap2a expression in premigratory neural crest in wild type (J) and a low mutant (K). (L) tfap2a expression in midline neural crest and in lateral mesoderm in the tailbud at nine somites (arrow). b, branchial; fb, forebrain; hb, hindbrain; h, hyoid; m, mandibular; mb, midbrain; nc, neural crest; nn, non-neural ectoderm; ot, otic vesicle; r1-7, rhombomeres; rb, Rohon-Beard cell; sc, spinal cord. Scale bars: 100 µm.

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