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Fig. 6. VAB-19 localization is dependent on VAB-10A/Plectin and Myotactin. VAB-19::GFP localization in attachment structure mutants examined using anti-GFP immunostaining. In let-805 (Myotactin) mutants, VAB-19::GFP localization is normal in early (1.5- to twofold stage) embryos (A); soon after the twofold stage, VAB-19::GFP dispersed into puncta throughout the dorsal and ventral epidermis (B). Live analysis of VAB-19::GFP in let-805 mutants revealed that this delocalization occurs within 15-20 minutes of the twofold stage (not shown). In vab-10A(ju281ts) animals grown at 25°C, VAB-19::GFP localization is normal at the 1.75-fold stage (C) but becomes disorganized after the twofold stage (D). Scale bar, 10 µm.





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