Fig. 1. Localization of HrPEM and macho 1 mRNAs in eggs, zygotes and embryos. mRNAs are visualized in fluorescent (A-D,F), confocal (A1-A3,B1-B3,C1-C3) and DIC microscopy (E,G), by fluorescent (TSA) (A-D,F) and chromogenic (E,G) methods. (A) Unfertilized egg (Unfert.). (B,C) Zygotes after first (Fert.20, 20 minutes after fertization) and second (Fert.80, 80 minutes after fertilization) major relocalization phases. (D,E) Lateral views of 8-cell embryos. (F,G) Animal-posterior views of 8-cell embryos. (A1,B1,C1) Median sections, the thickness of the labelled zone is 2 µm in unfertilized eggs and 5-8 µm in fertilized eggs. (A2-A3,B2-B3,C2-C3) Confocal tangential sections below the surface. Probes are indicated in the lower-left corner of each panel. a, animal; v, vegetal; p, posterior; CAB, centrosome-attracting body. Arrowheads in A,B,C show mRNA-rich cortical regions. Arrows in A2,B2 show that this mRNA-rich structure is reticulate.

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