Fig. 3. sap^ta222a is a mutation of dmd that causes fibre detachment and is phenocopied by knocking down dmd function. Dystrophin C-terminal immunoreactivity is localised to somite boundaries in wild type (WT) (A, 27 hours post-fertilisation, lateral views) but lacking in both sap (B) and dystrophin-morphant embryos (C). Evans blue dye (EBD, red), does not appear in WT somites (D), but labels fibres in both sap (E) and dystrophin-morphant embryos (F). Labelled fibres are visible that have both detached and retracted (arrowheads) or still span a somite and show a retraction zone (between arrows in F, lateral views, 72 hours post-fertilisation). By 72 hours post-fertilisation, dystrophin is present in the neural tube and notochord in both WT (G) and sap (J, asterisks) but lacking from muscle attachments in sap (arrowheads in G,J, transverse sections). ß-dystroglycan is also localised to muscle attachments (arrowheads) and other sites (asterisks) in WT (H), but unlike dystrophin is preserved at muscle attachments in sap (K, 72 hours post-fertilisation, transverse sections). Utrophin is not detectable at muscle attachments in either WT (I) or sap (L, arrowheads). Anti-utrophin immunoreactivity in the epidermis provides an internal positive control (asterisks).

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