Fig. 4. (A) Schematic representation of the DUET gene. The exons are indicated with black boxes. Arrows indicate the primers used for cDNA isolation and expression analysis. Coordinates are with respect to BAC F15E12. (B) The predicted sequence of DUET protein. The putative nuclear localization signal is in bold. The region showing homology to DYAD is underlined and the PHD-finger domain is boxed. The inverted triangle after amino acid 550 indicates the Ds transposon insertion site. (C) Analysis of DUET expression by RT-PCR. Expression of the DUET gene was examined in wild type (Wt) leaves (Lea), inflorescence (Inf) and duet mutant inflorescence by amplifying the cDNA synthesized from poly(A)⁺ mRNA. The shift in the size of the amplicon can be observed when genomic DNA (gen) was used as template. The constitutive GAPC gene was used as the normalization control. (D) Comparison of DUET and DYAD cDNA in pistils.

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