Fig. 1. Developmental profiles of Gcm gene expression in the mouse brain. (A) Messenger RNA was prepared from E16 placenta and brain, and used for RT-PCR analysis. Primers specific for mouse Gcm1, mouse Gcm2 and ß-actin were designed to recognize positions on different exons. (B) Total RNA was prepared from different developmental stages of the mouse brain, and used for quantification of mouse Gcm gene expression by real-time PCR analysis. Bars represent the relative mouse Gcm transcript levels normalized against ß-actin transcript levels.

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