Fig. 4. Effect of mouse Gcm1 expression during gliogenesis in vivo. Vibratome sections of P24 mouse forebrain that was injected with ß-gal-harboring retrovirus at E13 were stained with X-gal (A). B is a higher magnification of a box in A. The observed morphologies of infected cells were suggestive of neurons (arrows) and astrocytes (arrowheads). Neuron-like cells have a small clear cell body while astrocyte-like cells have a large obscure cell body. To confirm this classification, the cells were further stained (brown) with a neuronal marker, NeuN (C,D), or an astrocyte marker, GFAP (E,F). Using these criteria, the lineage of cells infected with CT or mouse Gcm1 retrovirus was examined (G). For mouse Gcm1-transduced cells, 69.9±7.2% of cells (n=2, 725 cells scored in total) were astrocytes while 33.6±10.2% (n=2, 1742 cells scored in total) of the cells were astrocytes for CT transduction. Scale bars: in A, 150 µm for A and 60 µm for B; in C, 25 µm for C,D; in E, 50 µm for E,F.

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