Fig. 3. Skeletal phenotype of the hindlimb. Dorsal view of right hindlimbs of wild-type, wild-type/raz and raz/raz embryos. Anterior is upwards in A-I and towards the left in J-L. Note the presence of an enlarged talus, the anterior bone in the proximal tarsus, in raz/raz hindlimbs (F,L), and the absence (F) or remnant (L) of a calcaneus, the posterior bone of the proximal tarsus. The distal end of the zeugopod of wild-type/wild-type hindlimbs has distinctive malleoli characteristic of the tibia and fibula (G), whereas the raz/raz hindlimb has malleoli characteristic of the anterior bone, the tibia (I). The bar of distal cartilage from which the peripheral digits arise in the autopod of the raz/raz limb (red arrow in L). Scale bars: in C, 1.0 mm for A-C; in F, 1.0 mm for D-F; in I, 1.0 mm for G-I; in L, 1.0 mm for K-L.

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