Fig. 8. Gli1 and Gli3 expression. (A-C) Whole-mount in situ hybridization to reveal Gli1 expression in the left forelimb of E11.5 wild-type/wild-type, wild-type/raz and raz/raz embryos. Anterior is upwards. Note the posterior expression of Gli1 in wild-type/wild-type limbs (A), and absence of expression in raz/raz limbs (C). (D-I) Gli3 expression in forelimbs (D-F) or left forelimb (G-I) of wild-type/wild-type, wild-type/raz and raz/raz embryos. Scale bar: 0.25 mm. (J) PCR analysis for Gli3 mRNA of E11.5 forelimbs (FL), hindlimbs (HL) and remainder of embryo (body) of wild-type/wild-type, wild-type/raz and raz/raz embryos. Limb buds were split longitudinally to provide anterior (Ant.) and posterior (Post.) samples. (K) Image quantitation of PCR analyses with wild-type/wild-type body given a value of 100 and other tissues normalized to that value. Results are mean±s.d.

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