|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Lack of any effect of CD8/MCSP on terminal differentiation or clonal growth
of primary human keratinocytes. (A) Flow cytometry of single cell suspensions
of primary human keratinocytes expressing empty retroviral vector, CD8 or
CD8/MCSP labelled with anti-involucrin antibody. The proportion of
involucrin-positive cells (indicated by the M1 marker) is indicated. (B)
Keratinocytes were plated in triplicate at clonal density onto mitotically
inactivated fibroblast feeders and cultured for 10-14 days. The proportion of
plated cells that formed clones of any size (%CFE) and the proportion of
clones that were abortive, consisting of terminally differentiated cells
(%Ab), were determined.
|
