Fig. 9. Effects of pharmacological inhibitors (A) and antibody crosslinking (B) on the actin cytoskeleton of keratinocytes expressing CD8 or CD8/MCSP. (A) Keratinocytes were incubated in medium alone (control) or supplemented with C3 toxin, Y-27632 or LY294002 to inhibit Rho, ROCK and phosphatidylinositol 3-kinase, respectively. (B) Keratinocytes were incubated with OKT8 alone (cont) or OKT8 in combination with anti-mouse IgG (X link) to induce crosslinking of the CD8 extracellular domain. Cells were labelled with TRITC-conjugated phalloidin. Scale bar, 40 µm.

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