Fig. 3. At23S::GFP expression pattern in abi3, fus3 and lec2 mutants. Seeds homozygous for the At2S3::GFP transgene were used to visualise the At2S3 promoter activity in wild-type Col-0 (A,E,I,S), abi3 (B,F,J,K,T), fus3 (D,H,L,M,V), lec2 (C,G,N-R,U). (A-D) Seeds harvested just before complete desiccation (yellow siliques, 15-16 DAP) visualised under white light. (E-H) Green fluorescence of the seeds shown in A-D. (I-R) Green fluorescence of isolated embryos extracted from seed coats at 11-13 DAP. All embryo images were taken with the same exposure except K,which is identical to picture J but exposed 10 times longer to visualise the low At2S3::GFP fluorescence present in abi3. (S-V) Green fluorescence signal present in the seed envelope at 16 DAP and arising from the endosperm layer. Scale bars: 500 µm (A-H) and 50 µm (I-W).

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