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Fig. 5. FUS3 and LEC2 promoter activities in embryos and
endosperm. LEC2::GUS (A-F) and FUS3::GUS (G-K) activities were assayed in
whole seeds (A,B,H,I: globular stages), isolated embryos (C,D, torpedo stages;
E, early mature embryos around 12 DAP; J, heart stage; K, mature embryo before
desiccation) or sections of seed envelopes (F,G). FUS3::GUS (M,O,Q) expression
pattern and At2S3::GFP green fluorescence (L,N,P) were compared in
individual lec2 mutant embryos just before desiccation (at 15-16
DAP). Scale bars: 50 µm. (R) Time course of FUS3 and LEC2
mRNA expression during silique development analysed by RT-PCR. The
developmental stage of samples is indicated in DAP. EF1
was
used as a control.
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