Fig. 4. Packaging defects and mispositioned oocytes in Cct1 mutant ovaries. (A-E) Egg chambers stained with Orb antibody (red) to label the oocyte, phalloidin (green) to visualize the actin cytoskeleton, and Hoechst (blue) to label the DNA. (A,D,F) Wild-type egg chambers. (C,G) Cct1^BN81 mutant egg chambers. (B,E) Cct1⁹⁹ mutant egg chambers. (A,D) Wild-type egg chambers consist of 15 nurse cells and an oocyte positioned at the posterior. (B) Cct1 mutant egg chambers have packaging defects, with multiple oocytes in one egg chamber. (C and E) Egg chambers from Cct1 mutant ovaries contain randomly positioned oocytes. Packaging defect and mispositioned oocyte phenotypes were also seen with other alleles. (F,G) Egg chambers labeled with antibody to Fas3 (green) to mark the polar follicle cells and Encore antibody (red) to label the oocyte. (F) Wild-type ovaries have a pair of polar follicle cells at each end of the egg chamber. (G) Polar follicle cells are present at both ends of mutant egg chambers with mispositioned oocytes. (H) Follicle cell clone mutant for Cct1¹⁷⁹ marked by absence of Myc protein expression (green). Asterisk indicates position of oocyte at posterior of egg chamber. (I,J) Egg chambers stained with antibody to Orb protein (red) to mark the oocyte, Hoechst (blue) to label the DNA, and GFP (green) to mark wild-type cells. (I) Egg chambers with complete follicle cell clones mutant for Cct1 (marked by absence of GFP expression in the follicle cell epithelium) showing mispositioned oocyte phenotype. (J) Egg chamber in which both the germline and follicle cell epithelium is mutant for Cct1 with a mispositioned oocyte phenotype.

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