Fig. 7. Mutations in Cct1 affect ovarian morphogenesis. (A-C) Figures adapted from King (King, 1970) depicting stages of ovarian morphogenesis at 2 hours, 24 hours and 36 hours APF. Apical cells are labeled in green, terminal filaments in purple, germline cells and follicle cell precursors in yellow and basal cells in red. (D-G) Pupal ovaries labeled with an antibody to Fas3 (red), which marks the basal cells and phalloidin (green) to visualize the actin cytoskeleton. (D) Wild-type ovary 20-22.5 hours APF with completely separated ovarioles and partially formed basal stalks. (E) Cct1⁹⁹ mutant ovary 20-22.5 hours APF. Apical cells have not migrated fully and basal stalks have not started to form. (F) Wild-type ovary 39-40 hours APF. Basal stalks have formed and become long and thin. (G) Cct1⁹⁹ mutant ovary 39-40 hours APF. Basal stalks have started to form but are incompletely separated. (H) Pupal ovary 20-21 hours APF with positively marked apical cell clones mutant for Cct1¹⁷⁹. Arrowheads indicate mutant cells, which are marked by expression of GFP (green). Ovaries are also stained with Fas3 antibody (red) to mark the basal stalks and phalloidin (blue) to visualize the actin cytoskeleton.

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