Fig. 1. Sxl is in the same complex as Ci. Extracts from wild-type 0-8 hour embryos treated with either anti-Sxl (A), anti-Ci rat monoclonal (B,C) or anti-Su(fu) antibodies (D), and the immunoprecipitates (IPs) tested for specific proteins by western blot analysis. (A) Sxl immunoprecipitates probed for Cos2, full-length Ci, Fu and Sxl. BicD was tested as a negative control for specificity of the immunoprecipitates. (B,C) Ci immunoprecipitates from OreR and Su(fu)^LP embryos, respectively, probed for Sxl and Fu. (Note the same result as in B was obtained using an anti-Ci amino-terminal polyclonal.) (D) Wild-type extract treated with Su(fu) antibodies probed for Sxl. Extract (E) shows the migration position of the relevant protein in an extract from OreR females on the same blot.

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