Fig. 6. Effect of Fu and Su(fu) on Sxl and the expression of Su(fu) in wild-type wing discs. (A-C) fu^mH63 disc stained for full-length Ci (red, A), Sxl (green, B) and merged image (C). The Sxl distribution is unaffected, but Ci shows the reported widened band at the AP border. (D,E) fu⁹⁴ discs stained for Sxl. Both show predominantly nuclear Sxl across the disc. The anterior compartment of the disc in D has a remnant of the nuclear gradient; lower levels of nuclear Sxl marked with an arrowhead. Line in D marks the approximate position of the AP border. (F) Su(fu)^LP disc stained for Sxl also shows the protein as nuclear across the entire disc. The disc was treated with only 5 ng/ml LMB for 2 hours. The fu⁹⁴ and fu^mH63 discs were treated with 50 ng/ml and 100 ng/ml LMB for 3 hours, respectively. (G-I). Su(fu) appears cytoplasmic and uniform across the disc. Ci protein (red, G) Su(fu) protein (green, H), merged image (I). As a control, Su(fu)^LP discs were simultaneously stained and these did not show any signal. Scale bar: 40 µm. All are confocal images; anterior is to the left, ventral at the top.

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