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Fig. 1. Brain and body axis defects in had mutants. (A,B) Live wild-type (left) and had mutant (right) embryo. The body axis is normal in had mutants at 24 hpf (A) but becomes curly by 48 hpf (B). (C,F) By 24 hpf, the boundary of midbrain and hindbrain is distinctive in wild-type embryos (C, arrow), but not had mutants (F, arrow). (D,G) Twoday-old wild-type (D) and had mutant embryos (G). Note that twoday-old had mutant has developed edema. (E,H) pax2 expression pattern in wild-type (E) and had mutant (H) embryos at 24 hpf.





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