|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Cardiomyocyte differentiation defects in had mutants. (A,B)
Transverse sections of two-day-old wild-type (A) and had mutant (B)
heart. Arrow indicates myocardium; arrowhead, endocardium. (C-H) Ventral views
of 50 hpf embryos, head to the top, solid line marks the site of the
constriction between the ventricle (v) and the atrium (a). Cardiac expression
of vmhc (C,D), irx1 (E,F) and versican (G,H) was
detected by in situ hybridization. The hearts of had mutants are
smaller and dysmorphic (B,D,F,H) compared with those in wild types (A,C,E,G).
By 50 hpf, expression of vmhc is restricted to ventricles in wild
types (C), but expression of this gene extends to the atrium in had
mutants (D). Cardiac expression of irx1 is restricted to ventricles
in wild-type embryos (E), but is severely reduced in the heart of had
mutants (F, arrow). versican is expressed at the boundary of the
atrium and the ventricle in wild-type embryos (G), but is expressed throughout
had mutant hearts (H).
|
