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Fig. 5. had encodes the zebrafish Na,K ATPase 
1B1 isoform. (A)
Linkage analysis shows 98 recombinants to z5508, 41 recombinants to z3705 and
no recombinants to Z6384 of LG1 in 848 meiosis. (B) Schematic cDNA and genomic
structures of zebrafish Na,KATPase 1B1. Dark boxes depict transmembrane
domains (TM1-TM10). Arrows indicate positions of primers (5F and 5R) used for
PCR analysis. Four exons (depicted by blue-boxes, labeled as exon A-D) were
amplified from the genomic DNA using the 5F and 5R primers. A deletion at the
3' boundary of exon C (red bar) was detected in had mutants.
(C) Genomic sequence of 1B1 between the 5F and 5R primer sequences. The
2 bp-insertion is shown in blue and sequences deleted in had are
shown in red. (D) RT-PCR analysis shows two alternative splicing variants
(arrows) in had mutants. (E) Predicted structure of the Na,K-ATPase
1B1 protein. Amino acids encoded by exon B are shown in green and those
encoded by exon C are in yellow. The purple region represents sequences
between TM7 and TM8, which include crucial elements for the interaction of the
and ß subunits. (F) The 1B1 isoform is highly expressed in
the two-day old wild-type embryonic heart, but is not detectable in
had mutant heart. Arrows point to the heart.
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