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Fig. 2. Spry1 is induced by FGF8b-soaked beads in prosomere 1 explants in
the absence of protein synthesis. Spry1 (A) and Spry2 (B)
are expressed in broad domains of the mid/hindbrain region at E9.5
Spry1 is induced by FGF8b-soaked beads within 4 hours of culture (D),
but not by BSA-soaked beads (C). (E-H) Spry1 is induced within 6
hours by FGF8 in the presence or absence of 50 µg/ml cyclohexamide (CHX) in
explants grown in medium containing 0.1% ethanol, but not by BSA-soaked beads.
Red arrows in D,F,H indicate induced gene expression. Green circles in C and H
indicate beads that were lost during processing of the tissues. Broken lines
in A and B indicate the tissues used for the explant assays.
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