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Fig. 5. FGF8b activates more molecular pathways than FGF8a, FGF17b and FGF18. (A-D) 1 µg/µl pMiw-Fgf8a induces Fgf8 (C), but does not induce Gbx2 (B) or Spry1 (D), or repress Otx2 (A). (E-H) 1 µg/µl pMiw-Fgf8b induces Gbx2 (F), Fgf8 (G) and Spry1 (H) and represses Otx2 (E). (I-L) 1 µg/µl pMiw-Fgf17b fails to induce Gbx2 (J), Fgf8 (K) or Spry1 (L), or to repress Otx2 (I). (M-P) 1 µg/µl pMiw-Fgf18 fails to induce Gbx2 (N), Fgf8 (O) or Spry1 (P), or to repress Otx2 (M). (Q-T) 1 µg/µl pMiw-caFGFR2 induces Gbx2 (R), Fgf8 (S) and Spry1 (T) in scattered cells. Otx2 is repressed on the electroporated side but scattered Otx2-expressing cells still exist (Q). In all panels, coronal or near coronal sections are shown with the anterior end towards the right. The broken lines indicate the midline with the electroporated side above the line and the control side below. In all panels, the red arrowheads indicate ectopic gene expression on the electroporated side and the green arrowheads indicate endogenous expression on the control side except for E and Q where the red arrowheads indicate the electroporated side where Otx2 expression is repressed (completely in E and incompletely in Q). Insets in A,C,E,G,I,M,Q show expression of the mouse or human genes electroporated into the right side of the brain.

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