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Fig. 8. FGF8b transforms the midbrain into a cerebellum and shifts the
mid/hindbrain organizer rostrally. (A) Experiments shown in B, C and D. 24
hours after 1 µg/µl pMiw-Fgf8b was electroporated into the
midbrain, Fgf8 expression is shifted into the caudal forebrain region
on the experimental side, as well as in a thin band along the dorsal midline
(red lines in A and arrowheads in B) that connects the ectopic Fgf8
domain to the endogenous Fgf8 domain on the control side. Green arrow
shows the down regulation of Fgf8 expression on the transfected side
in the isthmus. Inset shows the rear view of the same embryo. (C) 24 hours
after 1 µg/µl pMiw-Fgf8b is electroporated into the chicken
midbrain En1 expression is shifted rostrally on the electroporated
side, and seen in the most dorsal cells in the midbrain and anterior hindbrain
(red arrowheads), whereas the endogenous expression surrounding the isthmus
(green arrow) is downregulated. Inset shows a rear view of the same embryo,
note the normal expression on the control (left) side. (D) 24 hours after 1
µg/µl pMiw-Fgf8b is electroporated into the chicken midbrain,
the endogenous Wnt1 expression in the isthmus (green arrow) is
downregulated, whereas ectopic expression is induced near the dorsal midline
and in a transverse band in the rostral midbrain. Inset shows a rear view of
the same embryo, note the normal expression on the control (left) side. (E)
Scattered expression of Fgf8 is induced in the midbrain and caudal
forebrain (arrowheads) by ectopic expression of activated FGFR2. Note that the
endogenous Fgf8 expression is not repressed. (F) Scattered expression
of Wnt1 is induced in the midbrain and caudal forebrain (arrowheads)
by ectopic expression of activated FGFR2. Inset shows a rear view of the same
embryo.
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