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Fig. 2. The induction of early blood and endothelial progenitors occurs at the expense of the myocardial fate. (A-C and H,I) Flat mounts of embryos; anterior, top. (D-G and J,K) Anterior views of whole mounts; ventral, bottom. (L,M) Lateral views, anterior to the left. (A-C) At the 10-somite stage, nkx2.5 expression is reduced on the injected side in scl/tal1-lmo2 (SL)-injected embryos (C, arrow) but is perfectly normal in scl/tal1 (S)-injected (B) embryos. (D I) Likewise, gata4 (D,E), gata6 (F,G) and tbx5.1 (H,I) were reduced (E,G,I, arrows) on the side injected with scl/tal1-lmo2. (J-M) vmhc+ (Yelon et al., 1999) ventricular tissue was reduced at the 18-somite stage (J,K) and the nkx2.5+ heart tissue was reduced by 34 hpf (L,M) by injection with scl/tal1-lmo2.





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