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Fig. 3. scl/tal1-lmo2 coinjection induces erythropoiesis more anteriorly at the expense of the pronephros. (AE) Flat mounts. (F,G) Dorsal views of whole mounts; anterior, top. (H) Schematic of results. (A,B) scl/tal1-lmo2 injection (SL) increased the number of ßE1+ erythroid cells anteriorly and laterally, but they did not fuse at the midline. ßE1 was induced in non-neural ectoderm (B, asterisk). (C,D) In scl/tal1-lmo2-injected embryos, gata1 expression (purple) is broader and expanded anteriorly adjacent to somites 1-5 (arrows). (E-H) Scl/Tal1 and Lmo2 reduced expression of the pronephros (PN) marker wt1 (G), suggesting that erythroid development is expanded at the expense of the pronephros as shown in H.





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