|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Exogenous BMP4 downregulates the expression of Fgf8, Wnt5a and
cyclin D1 and suppresses cell proliferation. GTs from 12.0 dpc embryos were
cultured for 3 days (A,B) or for 24 hours (C-H) with BMP4 protein or BSA in
the medium. (A,B) BMP4-treated GT displayed retarded GT outgrowth. (C-H)
Fgf8, Wnt5a and cyclin D1 expression were suppressed by BMP4
treatment. Shh expression was unaltered by BMP4 treatment indicating
specific effects by BMP4 (data not shown). (I) Reduced cell proliferation of
BMP4-treated GT. The number of phospho-histone H3 immunopositive cells
decreased to 60% compared with those of control GT explants. (J) Alteration of
Fgf8 expression was observed 6.5 hours after the BMP4 treatment by
RT-PCR analysis. (A-H) Ventral views. Scale bar: 250 µm in A,B; 325 µm
in C-H.
|
